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pet 28a vectors in c43 de3 escherichia coli  (Lucigen Corp)

 
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    Lucigen Corp pet 28a vectors in c43 de3 escherichia coli
    Pet 28a Vectors In C43 De3 Escherichia Coli, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet-28a(+)+vectors/c41+coli+e/pm41055983-267-8-15
    Average 86 stars, based on 1 article reviews
    pet 28a vectors in c43 de3 escherichia coli - by Bioz Stars, 2026-09
    86/100 stars

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    other:

    Article Title: Design principles of the common Gly-X6-Gly membrane protein building block
    Article Snippet: SUMO-fusion constructs were cloned and expressed in pET-28a(+) vectors in C43(DE3) E. coli (Lucigen) grown in Terrific Broth (TB) with 50 μg/mL kanamycin induced with 0.5 mM IPTG at either 18, 30 or 37°C overnight, depending on the ideal temperature for that specific protein determined on a small scale.

    Construct:

    Article Title: Design principles of the common Gly-X6-Gly membrane protein building block.
    Article Snippet: .. SUMO- fusion constructs were cloned and expressed in pET- 28a(+) vectors in C43(DE3) Escherichia coli (Lucigen) grown in Terrific Broth (TB) with 50 μg/mL kanamycin induced with 0.5 mM IPTG at either 18, 30, or 37 °C overnight, depending on the ideal temperature for that specific protein determined on a small scale. .. Cells were resuspended, lysed by tip sonicate, and extracted with 20 mM C14- Betaine (C14B, Sigma) detergent, prior to high- speed centrifugation.

    Clone Assay:

    Article Title: Design principles of the common Gly-X6-Gly membrane protein building block.
    Article Snippet: .. SUMO- fusion constructs were cloned and expressed in pET- 28a(+) vectors in C43(DE3) Escherichia coli (Lucigen) grown in Terrific Broth (TB) with 50 μg/mL kanamycin induced with 0.5 mM IPTG at either 18, 30, or 37 °C overnight, depending on the ideal temperature for that specific protein determined on a small scale. .. Cells were resuspended, lysed by tip sonicate, and extracted with 20 mM C14- Betaine (C14B, Sigma) detergent, prior to high- speed centrifugation.



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